Kat Pick
Microbiology PhD Candidate, University of Alberta
Phage-inducible chromosomal island aids helper phage replication (ISME, 2025)
Phage-inducible chromosomal island aids helper phage replication (ISME, 2025)
Complete genome sequence of E. coli MP1 (MRA, 2024)
Complete genome sequence of E. coli MP1 (MRA, 2024)
Escherichia coli MP1 was isolated from the feces of a healthy CD-1 mouse by selective plating on MacConkey lactose agar, then screening for beta-glucuronidase activity on LB agar containing X-Gluc, and sensitivity to phage P1vir by spot titer (1). MP1 has been used as a model system to study E. coli colonization of the mouse gastrointestinal tract in numerous publications. The available assembly, generated by 454 pyrosequencing, consists of 84 contigs (1). We thus aimed to provide a completed genome assembly for this important model organism. MP1 was generously provided to us by the Goulian Lab (University of Pennsylvania) shipped at ambient temperature on filter paper, submerged in 5 mL LB broth upon receipt, and grown overnight at 37°C 225 rpm aerobically. Furthermore, 0.5 mL of this culture was used to create a stock in 25% glycerol, maintained at −80°C.
Bacterial envelope stress responses review (BBA, 2023)
Bacterial envelope stress responses review (BBA, 2023)
Isolation and characterization of Kapi1 (mBio, 2022)
Isolation and characterization of Kapi1 (mBio, 2022)
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