FlatMux is an optimized microscope system for multiplexed two-photon imaging of neuronal activity with genetically encoded voltage sensors. The flexible system is demonstrated in a variety of scenarios in the mouse brain including a large field of view, deep imaging or two-plane imaging.

A little more than one year ago, Nature Methods rolled out a program to formally recognize early-career researcher contributions to our peer review process. Here we provide an update on the program’s success.

Biologists can reuse, remix and vibe code scientific figures.

Two scientists blend their love of music and science in different ways.

MemBrain v2 enables streamlined analysis of membranes in cryo-electron tomography by providing user-friendly tools for membrane segmentation, membrane protein localization and spatial analysis, supporting workflows across diverse datasets.

IRIS learns conserved signaling representations from in vitro perturbation data to reconstruct signaling states and histories across diverse in vivo cellular contexts.

DeepWonder3D is a pipeline for neuronal extraction from volumetric calcium imaging data acquired with a variety of one-photon or two-photon microscopy modalities.

Spl-ISO-Seq2, Spl-IsoQuant-2 and Spl-IsoFind enable isoform sequencing, barcode calling of >450 million barcodes, and spatially variable isoform detection with high spatial resolution as demonstrated on mouse brain slices.

Iterative Mapping of proteoforms enables large-scale measurement of intact proteoforms with single-molecule resolution. The approach is demonstrated here by quantifying tau proteoform groups across control samples of known composition, model systems used in tauopathy research, and human-derived brain tissue samples.

Spatial-ATAC-Hi-C enables the profiling of both chromatin accessibility and organization in a spatially resolved manner, as demonstrated on mouse brain and human glioblastoma and astrocytoma samples.

Many have dreamt of studying and getting their scientific training in the United States. These days, that dream is no easy pursuit.

Either I’ve been missing something or nothing has been going on. —Karen Elizabeth Gordon