A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes of various organisms.

Using electron microscopy, scientists mapped a Caenorhabditis elegans nervous system and Drosophila brain at single-neuron resolution. Connectomics work on bigger brains takes new methods strategies.

Connectomics has so far outpaced the Moore’s law predictions about technological progress. At this pace, many whole-brain connectomes and connectomic screening will soon be realistic.

CABLE harnesses cytoarchitectural information such as cell or nuclear shape to infer fiber tracts in the brain of, for example, marmosets, macaques or humans.

DynamicAtlas integrates fixed and live imaging data to generate a morphodynamic atlas of Drosophila development.

iGluSnFR4f and iGluSnFR4s are the latest generation of genetically encoded glutamate sensors. They are advantageous for detecting rapid dynamics and large population activity, respectively, as demonstrated in a variety of applications in the mouse brain.

This study advances methods for benchmarking macromolecular complex predictions by introducing a scalable open-source framework used in recent community assessments to compare structures, interfaces and ligand interactions against experimental data.

CELTIC is a context-dependent deep learning model for in silico organelle localization that uses biological priors to enable the generalized and harmonized prediction of organelle fluorescence from label-free images.

SpaceBar is a cellular barcoding strategy for simultaneous analysis of cell clonal and spatial identities.

The SpatialEx(+) framework uses histology to generate and integrate complementary spatial multi-omics data across samples.

Methods to study the structural and functional properties of proteins that contain intrinsically disordered regions at the proteome scale are on the rise.

Protein editors complement genome editing tools by enabling direct modifications to protein molecules.

Extracellular recordings provide a wealth of information about neuronal activity but it is difficult to link this information to cell types.

New technologies and computational approaches will illuminate the role of the cancer microbiome.