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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
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2,294 4,450
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
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361 832
News
News about the latest advances and discoveries in sequencing and related technologies.
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404 411
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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128 581
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 1,927 Posts: 7,103
1,927 7,103
Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
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50 66
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 1,536 Posts: 2,216
1,536 2,216
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51 Posts: 163
51 163
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2 Posts: 5
2 5
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,067 Posts: 16,767
3,067 16,767
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 388 Posts: 2,175
Last Post: Proton Data
388 2,175
ehaag
by ehaag
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 124 Posts: 608
124 608
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 389 Posts: 2,182
389 2,182
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0 Posts: 0
0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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0 0
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 78 Posts: 288
78 288
Miranda
by Miranda
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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75 477
Archived Forums
A place for discussion of older forums and sequencing technologies.
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Last Post: Rapid Libraries
1,361 7,278
pmiguel
by pmiguel
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UK - Cambridge (37/67)
The Pipeline (81/587)
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454 Pyrosequencing (743/3,966)
Polonator (6/65)
Bioinformatics
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 22,066 Posts: 103,093
22,066 103,093
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
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803 1,326
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Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
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vivianshaw
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
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1,558 1,777
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,199 Posts: 9,921
2,199 9,921
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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8 15
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 280 Posts: 994
280 994
De novo discovery
Wandering without a reference? Post here
Topics: 340 Posts: 1,699
340 1,699
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 268 Posts: 847
268 847
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357 Posts: 1,578
357 1,578
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,864 Posts: 11,427
2,864 11,427
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 38 Posts: 104
38 104
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 60 Posts: 294
60 294
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Announcements regarding SEQanswers
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seqadmin
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  • SEQadmin2
    Population Genomics: Applications in Healthcare, Conservation, Agriculture, and More

    Foundation, co-evolution with NGS, and impact

    Population genomics is the use of DNA data from entire populations to understand the genetic makeup of a group of individuals, human or otherwise. We previously covered the basics of population genomics and how next-generation sequencing (NGS) expanded the field here.1 Since then, population genomics has co-evolved with NGS to achieve measurable impacts in several areas.

    This article will cover:

    ● How
    ...
    03-09-2026, 06:20 AM
  • SEQadmin2
    Decoding the Tumor Microenvironment with Next-Generation Sequencing


    Cancer is increasingly understood as an ecosystem in which tumor cells co-evolve with immune, stromal, and vascular components of the surrounding tumor microenvironment (TME). These interactions affect disease progression, therapeutic response, and the emergence of resistance. Yet, the molecular mechanisms underlying these processes have long been difficult to resolve. Next-generation sequencing (NGS) technologies now enable high-resolution analysis of this dynamic landscape. Whole-genome...
    02-23-2026, 11:07 AM
  • SEQadmin2
    NGS Advances as Tool for Synthetic Biology DBTL Cycle





    Synthetic biology has advanced quickly in the last decade, and a lot of that is because of next-generation sequencing (NGS). In synthetic biology, engineering principles are applied to biological systems, to modify or create new biological organisms or molecules. Because of this, the working paradigm is the design-build-test-learn (DBTL) cycle.

    With the instructions to every organism, system, or molecule being encoded in DNA, NGS is crucial for all
    ...
    02-13-2026, 10:34 AM

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