Jonathan Weissman's Lab (@JswLab) / X

Jonathan Weissman's Lab

176 posts

Jonathan Weissman's Lab

@JswLab

Jonathan S. Weissman's lab at the @WhiteheadInst/@MIT. Account run by lab members.

Cambridge, MA

Joined October 2019

Jonathan Weissman's Lab
@JswLab
Jan 18, 2020
Gene expression levels are continuous rather than binary. To access biological phenotypes along such a continuum, we developed a method to titrate gene expression using systematically attenuated CRISPR guide RNAs. Check out our work in @NatureBiotech
Nature Biotechnology
@NatureBiotech
Jan 13, 2020
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs go.nature.com/35QGI6f
Jonathan Weissman's Lab
@JswLab
Mar 30, 2020
Perturb-seq is a powerful tool for understanding genes and genetic networks at the single-cell level. We developed a method for large-scale Perturb-seq screens with flexible guide designs and targeted sequencing. Check out our work in @NatureBiotech:
Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
From nature.com
Jonathan Weissman's Lab
@JswLab
Jun 15, 2021
Have you ever wondered exactly what happens when Cas9 creates a double-strand break? We're excited to share Repair-seq, an approach for screening how thousands of genetic perturbations change the mutations that cells make when they repair DNA damage.
biorxiv.org
Mapping the Genetic Landscape of DNA Double-strand Break Repair
Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways that are critical for maintaining genomic integrity. Here we present Repair-seq, a high-throughput screening approach...
Jonathan Weissman's Lab
@JswLab
Oct 20, 2021
Out now in @CellCellPress, we report the development of Repair-seq, a high-throughput screening approach for systematically mapping DNA repair pathways.
Mapping the genetic landscape of DNA double-strand break repair
From cell.com
Jonathan Weissman's Lab
@JswLab
Oct 4, 2019
The Weissman lab finally has a public twitter feed!
Jonathan Weissman's Lab
@JswLab
Oct 13, 2021
We are so excited to share the latest from the lab (joint with @YosefLab and @LabJacks ), where we engineer an evolving CRISPR-based, single-cell lineage tracer into a mouse model of lung cancer and monitor tumor growth and evolution with unprecedented resolution.
Matt Jones
@MattG_Jones
Oct 13, 2021
We're ecstatic to present our manuscript on recording the evolutionary dynamics in a mouse model of lung cancer with an evolving lineage tracer. Congrats to @dianyang1117 for co-leading and @JswLab @YosefLab @LabJacks for this joint effort. Biorxiv link: tinyurl.com/f43de4nw
Jonathan Weissman's Lab
@JswLab
Dec 24, 2020
Check out our latest pre-print describing a strategy for CRISPR screens in human DCs and its use to probe how DCs recognize immune ligands (LPSs) from the microbome:
biorxiv.org
CRISPR-based functional genomics in human dendritic cells
Dendritic cells (DCs) regulate processes ranging from antitumor and antiviral immunity to host-microbe communication at mucosal surfaces. It remains difficult, however, to genetically manipulate...
Jonathan Weissman's Lab
@JswLab
Oct 26, 2021
Check out our latest paper in @NatureBiotech on functional single-cell genomics of HCMV infection! @marcoyannic used Perturb-seq to study how the course of infection changes when we target host factors or critical viral genes.
Nature Biotechnology
@NatureBiotech
Oct 25, 2021
Functional single-cell genomics of human cytomegalovirus infection go.nature.com/3jBQtyJ
Jonathan Weissman's Lab
@JswLab
Apr 20, 2020
Past events in cancer progression, like metastasis, leave clear signatures in cancer cells' ancestry. Using our CRISPR-based single-cell lineage tracer, we explored the rates, routes, and patterns of metastatic spread in a lung cancer mouse model. Check out our new preprint 👇
bioRxiv
@biorxivpreprint
Apr 17, 2020
Continuous lineage recording reveals rapid, multidirectional metastasis in a lung cancer xenograft model in mouse biorxiv.org/cgi/content/sh… #bioRxiv
Jonathan Weissman's Lab
@JswLab
Apr 30, 2021
We are currently hiring technicians through HHMI & the Whitehead Institute! Please encourage motivated undergrads to apply!
Jonathan Weissman's Lab
@JswLab
Aug 22, 2023
Excited to announce the pre-print (biorxiv.org/content/10.110…) for our work studying the biogenesis pathways of mitochondrial outer membrane alpha-helical proteins! A really fun collaboration with the (@voorheeslab). Follow this 🧵 for a sneak peak (1/12)
biorxiv.org
Triaging of ⍺-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery...
Mitochondrial outer membrane ⍺-helical proteins play critical roles in mitochondrial-cytoplasmic communication, but the rules governing the targeting and insertion of these biophysically diverse...
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Jonathan Weissman's Lab
@JswLab
Dec 18, 2021
Out now on biorxiv, check out our genome-scale Perturb-seq manuscript! Check out this thread from @josephmreplogle for more details
Joseph Replogle
@josephmreplogle
Dec 18, 2021
I'm thrilled to share our latest work performing genome-scale Perturb-seq screens to explore a remarkable breadth of questions in genetics and cell biology (thread below). Congrats to Reuben Saunders, Tom Norman, and @JSWlab for co-leading this work. biorxiv.org/content/10.110…
Jonathan Weissman's Lab
@JswLab
Nov 21, 2024
In collaboration with @weallen1 and Xiaowei Zhuang, we have developed Perturb-multi:
William Allen
@weallen1
Nov 21, 2024
In collaboration with Reuben Saunders, @JswLab, and Xiaowei Zhuang, we are very excited to release Perturb-Multi: a platform for pooled multimodal genetic screens in intact mammalian tissue. Check it out! biorxiv.org/content/10.110…
biorxiv.org
A platform for multimodal in vivo pooled genetic screens reveals regulators of liver function
Organ function requires coordinated activities of thousands of genes in distinct, spatially organized cell types. Understanding the basis of emergent tissue function requires approaches to dissect...
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Jonathan Weissman's Lab
@JswLab
Mar 14, 2024
Glad to have our work using natural barcode-based lineage tracing to decipher cell states and genealogies of human hematopoiesis out today in @Nature, led by @chenweng1991, a great collaboration with @bloodgenes. doi.org/10.1038/s41586… Check out this terrific thread as well!
Chen Weng
@chenweng1991
Jan 23, 2024
Very delighted to share our work Deciphering cell states and genealogies of human hematopoiesis online today in @Nature (Accelerated Preview version). It is a really great collaboration of Weissman lab @JswLab and Sankaran lab @bloodgenes doi.org/10.1038/s41586… 🧵(1/n)
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